Insect Pathology and Pathogen Genomics Lab.
National Chung Hsing University
Our papers
A new nucleopolyhedrovirus strain (LdMNPV-like virus) with a defective fp25 gene from Lymantria xylina (Lepidoptera: Lymantriidae) in Taiwan
Yu-Shin Nai, Tai-Chuan Wang, Yun-Ru Chen, Chu-Fang Lo, Chung-Hsiung Wang
J. Invertebr. Pathol. 102, 110-119 (2009).
Honey Bee Colony Collapse Disorder
Yu-Shin Nai, Chih-Yu Wu, Tai-Chuan Wang, Yun-Ru Chen, Wei-Hong Lau, Chu-Fang Lo, Meng-Feng Tsai, Chung-Hsiung Wang
Formosan Entomol. 29, 119-138 (2009).
Genomic sequencing and analyses of Lymantria xylina multiple nucleopolyhedrovirus
Yu-Shin Nai, Chih-Yu Wu, Tai-Chuan Wang, Yun-Ru Chen, Wei-Hong Lau, Chu-Fang Lo, Meng-Feng Tsai, Chung-Hsiung Wang
BMC Genomics. 11, 116 (2010).
A new microsporidium, Triwangia caridinae gen. nov., sp. nov. parasitizing fresh water shrimp, Caridina formosae (Decapoda: Atyidae) in Taiwan
Tai-Chuan Wang, Yu-Shin Nai, Chih-Yuan Wang, Leellen F Solter, Hui-Chen Hsu, Chung-Hsiung Wang, Chu-Fang Lo
J. Invertebr. Pathol. 112(3), 281-293 (2013).
A new spiroplasma isolate from the field cricket (Gryllus bimaculatus) in Taiwan
Yu-Shin Nai, Ping-Yi Su, Yu-Hsiang Hsu, Ching-Hao Chiang, Jae Su Kim, Yue-Wen Chen, Chung-Hsiung Wang
J. Invertebr. Pathol.120, 4-8 (2014).
Management of Entomopathogenic Fungi in Cultures of Tenebrio molitor (Coleoptera: Tenebrionidae)
Se Jin Lee, Si Hyeon Kim, Yu‐Shin Nai, Yeon Ho Je, Bruce L Parker, Jae Su Kim
Entomol. Res. 44(6), 236-243 (2014).
Beauveria bassiana granules for management of brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae) in rice
Se Jin Lee, Jeong Seon Yu, Yu-Shin Nai, Bruce L Parker, Margaret Skinner, Jae Su Kim
BioControl. 60, 263-270 (2014).
Biological control of Asian tiger mosquito, Aedes albopictus (Diptera: Culicidae) using Metarhizium anisopliae JEF-003 millet grain
Se Jin Lee, Sihyeon Kim, Jeong Seon Yu, Jong Cheol Kim, Yu-Shin Nai, Jae Su Kim
J. Asia-Pac. Entomol. 18(2), 217-221 (2015).
Expression of egfp gene based on Agrobacterium tumefaciens-mediated transformation in Beauveria bassiana sensu lato ERL836
Yu-Shin Nai, Se Jin Lee, Sihyeon Kim, Ho-Jong Ju, Yeon Ho Je, Jae Su Kim
J. Asia-Pac. Entomol. 18(4), 677-681 (2015).
Aedia leucomelas (Lepidoptera: Noctuidae)-Pathogenic Entomophaga aulicae (Zygomycetes: Entomophthorales) in Sweet Potato Fields
Seon-Wu Choi, Ju-Rak Lim, Hyung-Cheol Moon, Ju-Hee Kim, Young-Ju Song, Yu-Shin Nai, Jae Su Kim
J. Asia-Pac. Entomol. 19(1), 71-79 (2016).
Baculoviral IAP2 and IAP3 encoded by Lymantria xylina multiple nucleopolyhedrovirus (LyxyMNPV) suppress insect cell apoptosis in a transient expression assay
Yu-Shin Nai, Yi-Ting Yang, Jae Su Kim, Chih-Yu Wu, Yue-Wen Chen, Chung-Hsiung Wang
Appl. Entomol. Zool. 51(2), 305-316 (2016).
The impact of pyriproxyfen on the development of honey bee (Apis mellifera L.) colony in field
Yue-Wen Chen, Pei-Shan Wu, En-Cheng Yang, Yu-Shin Nai, Zachary Y Huang
J. Asia-Pac. Entomol. 19(3), 589-594 (2016).
Characterization of T-DNA insertion mutants with decreased virulence in the entomopathogenic fungus Beauveria bassiana JEF-007
Sihyeon Kim, Se Jin Lee, Yu-Shin Nai, Jeong Seon Yu, Mi Rong Lee, Yi-Ting Yang, Jae Su Kim
Appl. Microbiol. Biotechnol. 100(20), 8889-8900 (2016).
Up-regulation of carbon metabolism-related glyoxylate cycle and toxin production Beauveria bassiana JEF-007 during infection of bean bug, Riptortus pedestris (Hemiptera: Alydidae)
Yi-Ting Yang, Se Jin Lee, Yu-Shin Nai, Sihyeon Kim, Jae Su Kim
Fungal biol. 120(10), 1236-1248 (2016).
A novel picorna-like virus, Riptortus pedestris virus-1 (RiPV-1), found in the bean bug, R. pedestris, after fungal infection
Yi-Ting Yang, Yu-Shin Nai, Se Jin Lee, Mi Rong Lee, Sihyeon Kim, Jae Su Kim
J. Invertebr. Pathol. 141, 57-65 (2016).
T Oligo-primed polymerase chain reaction (TOP-PCR): A robust method for the amplification of minute DNA fragments in body fluids
Yu-Shin Nai, Tzu-Han Chen, Yu-Feng Huang, Mohit K Midha, Hsin-Chieh Shiau, Chen-Yang Shen, Chien-Jen Chen, L Yu Alice, Kuo Ping Chiu
Sci. Rep. 7(1), 1-12 (2017).
Evaluating the Effect of Environmental Chemicals on Honey Bee Development from the Individual to Colony Level
Chong-Yu Ko, Yue-Wen Chen, Yu-Shin Nai
J. Vis. Exp. 122, e55296 (2017).
Determination of nucleopolyhedrovirus’ taxonomic position
Yu-Shin Nai, Yu-Feng Huang, Tzu-Han Chen, Kuo-Ping Chiu, Chung-Hsiung Wang
Biological Control of Pest and Vector Insects. (2017): Chapter 8: p169-p200. InTech publisher, ISBN 978-953-51-3035-2.
Tenebrio molitor Gram‐negative‐binding protein 3 (TmGNBP3) is essential for inducing downstream antifungal Tenecin 1 gene expression against infection with Beauveria bassiana JEF‐007
Yi-Ting Yang, Mi Rong Lee, Se Jin Lee, Sihyeon Kim, Yu-Shin Nai, Jae Su Kim
Insect Sci. 8(1), 1-12 (2017).
Revealing pesticide residues under high pesticide stress in Taiwan’s agricultural environment probed by fresh honey bee pollen
Yu-Shin Nai, Tsui-Yao Chen, Yi-Cheng Chen, Chun-Ting Chen, Bor-Yann Chen, Yue-Wen Chen
J. Econ. Entomol. 110 (5), 1947-1958 (2017).
Relationship between expression level of hygromycin B resistant gene and Agrobacterium tumefaciens-mediated transformation efficiency in Beauveria bassiana JEF-007
Yu‐Shin Nai, Mi Rong Lee, Sihyeon Kim, Se Jin Lee, Yi‐Ting Yang, Jae Su Kim
J. Appl. Microbiol. 123(3), 724-731 (2017).
Tenebrio molitor-mediated entomopathogenic fungal library construction for pest management
Jong Cheol Kim, Mi Rong Lee, Sihyeon Kim, Se Jin Lee, So Eun Park, Yu-Shin Nai, Gwan Seok Lee, Tae Young Shin, Jae Su Kim
J. Asia-Pac. Entomol. 21(1), 196-204 (2018).
Transient expression of foreign genes in insect cells (sf9) for protein function assay
Ju-Chun Chang, Se Jin Lee, Jae Su Kim, Chung-Hsiung Wang, Yu-Shin Nai
J. Vis. Exp. 132, e56693 (2018).
The seasonal detection of AcSBV (Apis cerana sacbrood virus) prevalence in Taiwan
Yu-Shin Nai, Chong-Yu Ko, Pei-Shou Hsu, Wen-Shi Tsai, Yue-Wen Chen, Meng-Hao Hsu, I-Hsin Sung
J. Asia-Pac. Entomol. 21(1), 417-422 (2018).
Characterization and functional assay of apsup (Lyxy-105) from Lymantria xylina multiple nucleopolyhedrovirus (LyxyMNPV)
Ju-Chun Chang, Zih-Ting Chang, Yu-Feng Huang, Se Jin Lee, Jae Su Kim, Yu-Shin Nai
Virus Genes 54(4), 275-586 (2018).
Genomic Analysis of the Insect-Killing Fungus Beauveria bassiana JEF-007 as a Biopesticide
Se Jin Lee, Mi Rong Lee, Sihyeon Kim, Jong Cheol Kim, So Eun Park, Dongwei Li, Tae Young Shin, Yu-Shin Nai, Jae Su Kim
Sci. Rep. 8 (1), 1-12 (2018).
Transcriptional response of bean bug (Riptortus pedestris) upon infection with entomopathogenic fungus, Beauveria bassiana JEF-007
Se Jin Lee, Yi‐Ting Yang, Sihyeon Kim, Mi Rong Lee, Jong Cheol Kim, So Eun Park, Muktadir S Hossain, Tae Young Shin, Yu‐Shin Nai, Jae Su Kim
Pest Manag. Sci. 75(2), 333-345 (2019).
Mechanistic insight into the attenuation of gouty inflammation by Taiwanese green propolis via inhibition of the NLRP3 inflammasome
Chih‐Yu Hsieh, Lan‐Hui Li, Yerra Koteswara Rao, Tz‐Chuen Ju, Yu‐Shin Nai, Yue‐Wen Chen, Kuo‐Feng Hua
J. Cell. Physiol. 234(4), 4081-4094 (2019).
Genomic sequencing of Troides aeacus nucleopolyhedrovirus (TraeNPV) from golden birdwing larvae (Troides aeacus formosanus) to reveal defective Autographa californica NPV genomic features
Yu-Feng Huang, Tzu-Han Chen, Zih-Ting Chang, Tai-Chuan Wang, Se Jin Lee, Jong Cheol Kim, Jae Su Kim, Kuo-Ping Chiu, Yu-Shin Nai
BMC Genom. 20(1), 1-16 (2019).
A newly designed EGFP-2A peptide monocistronic baculoviral vector for concatenating the expression of recombinant proteins in insect cells
Chih-Yu Wu, Chao-Wei Huang, Yu-Shin Nai, Pei-Yu Chu, Chung-Hsiung Wang, Shih-Torng Ding
Processes 7(5), 291 (2019).
Dynamics of Apis cerana Sacbrood Virus (AcSBV) Prevalence in Apis cerana (Hymenoptera: Apidae) in Northern Taiwan and Demonstration of its Infection in Apis mellifera (Hymenoptera: Apidae)
Chong-Yu Ko, Zong-Lin Chiang, Ruo-Jyun Liao, Zih-Ting Chang, Ju-Chun Chang, Tsun-Yung Kuo, Yue-Wen Chen, Yu-Shin Nai
J. Econ. Entomol. 112(5), 2055-2066 (2019).
Downstream processing of Beauveria bassiana and Metarhizium anisopliae-based fungal biopesticides against Riptortus pedestris: solid culture and delivery of conidia
Min Ho Song, Jeong Seon Yu, Sihyeon Kim, Se Jin Lee, Jong Cheol Kim, Yu-Shin Nai, Tae Young Shin, Jae Su Kim
Biocontrol Sci. Technol. 29(6), 514-532 (2019).
Novel inspection of sugar residue and origin in honey based on the 13C/12C isotopic ratio and protein content
Chun-Ting Chen, Bor-Yann Chen, Yu-Shin Nai, Yuan-Mou Chang, Kuan-Hua Chen, Yue-Wen Chen
J. Food Drug Anal. 25 (6), 969-977 (2019).
Diversity of fungal DNA methyltransferases and their association with DNA methylation patterns
Yu-Shin Nai, Yu-Chun Huang, Ming-Ren Yen, Pao-Yang Chen
Front. Microbiol. 11, 3614 (2020).
Screening of Differentially Expressed Microsporidia Genes from Nosema ceranae Infected Honey Bees by Suppression Subtractive Hybridization
Zih-Ting Chang, Chong-Yu Ko, Ming-Ren Yen, Yue-Wen Chen, Yu-Shin Nai
Insects 11(3), 199 (2020).
Complete genome sequence of a novel putative RNA virus, RiPV-2, from the Bean Bug Riptortus pedestris
Yi-Ting Yang, Se Jin Lee, Yu-Shin Nai, Jae Su Kim
Microbiol. Resour. Announc. 9(21), e01584-19 (2020).
Persistent PnV (Perina nuda virus) infection in a heterologous Lymantria xylina cell line, NTU-LY
Yu-Shin Nai, Chou-Ming Lo
Biocontrol Sci. Technol. 30(9), 929-940 (2020).
Deformed Wing Virus in Two Widespread Invasive Ants: Geographical Distribution, Prevalence, and Phylogeny
Chun-Yi Lin, Chih-Chi Lee, Yu-Shin Nai, Hung-Wei Hsu, Chow-Yang Lee, Kazuki Tsuji, Chin-Cheng Scotty Yang
Viruses 12 (11), 1309 (2020).
Comparison of gut microbiota of healthy and diseased walking sticks, Phasmotaenia lanyuhensis
Yi‐Hsuan Li, Yu‐Feng Huang, Tzu‐Han Chen, Shin‐Shan Wu, Hsin‐Chieh Tang, Chung‐Yi Hsiao, Lung‐Chun Huang, Ju‐Chun Chang, Kuo‐Ping Chiu, Yu‐Shin Nai
Arch. Insect Biochem. Physiol. 105(4), e21749 (2020).
Genomic Sequencing and Comparison of Sacbrood Viruses from Apis cerana and Apis mellifera in Taiwan
Ju-Chun Chang, Zih-Ting Chang, Chong-Yu Ko, Yue-Wen Chen, Yu-Shin Nai
Pathogens 10(1), 14 (2021).
Construction and selection of an entomopathogenic fungal library from soil samples for controlling Spodoptera litura
Ju-Chun Chang, Shin-Shan Wu, Yao-Chia Liu, Yu-Hong Yang, Yi-Fang Tsai, Yi-Hsuan Li, Ching-Tzu Tseng, Li-Cheng Tang, Yu-Shin Nai
Front. Sustain. Food Syst. 5, 15 (2021).
Transcriptome-level assessment of the impact of deformed wing virus on honey bee larvae
Zih-Ting Chang, Yu-Feng Huang, Yue-Wen Chen, Ming-Ren Yen, Po-Ya Hsu, Tzu-Han Chen, Yi-Hsuan Li, Kuo-Ping Chiu, Yu-Shin Nai
Scientific reports 11 (1), 1-13 (2021).